Catalog No: OKCD07833
Size:96WELLS
Price: $750.00
SKU
OKCD07833
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Datasheets/ManualsPrintable datasheet for TARDBP ELISA Kit (Human) (OKCD07833)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tar DNA Binding Protein 43kDa (TDP43). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tar DNA Binding Protein 43kDa (TDP43). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tar DNA Binding Protein 43kDa (TDP43), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tar DNA Binding Protein 43kDa (TDP43) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range62.5-4,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tar DNA Binding Protein 43kDa (TDP43) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tar DNA Binding Protein 43kDa (TDP43) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
TARDBP Microplate96 Wells (12 x 8 Well strips)
TARDBP Lyophilized Standard2
100X TARDBP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, cerebrospinal fluid and other biological fluids
Sensitivity< 24.6pg/mL
Gene SymbolTARDBP
Gene Full NameTAR DNA binding protein
Alias SymbolsALS10;TAR DNA-binding protein 43;TAR DNA-binding protein-43;TDP-43.
NCBI Gene Id23435
Protein NameTAR DNA-binding protein 43
Description of TargetDNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
Uniprot IDQ13148
Protein Accession #NP_031401.1
Nucleotide Accession #NM_007375.3
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