TARDBP Antibody (OAPB00552)

Catalog No: OAPB00552

Size:100UG

Western blot analysis of TDP43 in HeLa cell lysate with TDP43 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunofluorescence of TDP43 in Hela cells with TDP43 antibody at 20 ug/mL.

Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Chicken|Human|Mouse|Rat
• Product Format: Liquid. TDP43 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: TDP43 antibody can be stored at 4°C for three months and-20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: TDP43 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TDP43. The immunogen is located within amino acids 60 - 110 of TDP43.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Chicken (100%)
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-TARDBP (OAPB00552) antibody is Catalog # OEPB02674
• Specificity: At least two isoforms are known to exist for this protein; this TDP43 antibody will recognize both isoforms.
• Application Info: WB 0.5~2 ug/ml
  ICC 5 ug/ml
  IF 20 ug/ml
• Other Applications Image 1 Data: Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 ug/mL.
• Reference: 1. Ou SH, Wu F, Garcia-Martinez LF, et al. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J. Virol.1995; 69:3584-96.;
  2. Buratti E, Dork T, Zuccato E, et al. Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping. EMBO J.2001; 20:1774-84.;
  3. Neumann M, Sampathu DM, Kwong LK, et al. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 2006; 314:42-3.;

Target Info

• Gene Symbol: TARDBP
• Gene Full Name: TAR DNA binding protein
• Alias Symbols: ALS10;TAR DNA-binding protein 43;TAR DNA-binding protein-43;TDP-43.
• NCBI Gene Id: 23435
• Protein Name: TAR DNA-binding protein 43|TDP43 [Homo sapiens]
• Description of Target: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
• Uniprot ID: Q13148
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/ABO32290
• Nucleotide Accession #: NM_007375.3