TARDBP Antibody (OAGA08475)

Catalog No: OAGA08475

Size:100 ul

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT733] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

TARDBP antibody [GT733] detects TARDBP protein by western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT733] dilution: 1:1000

TARDBP antibody [GT733] detects TARDBP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TARDBP protein stained by TARDBP antibody [GT733] diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: GT733
• Isotype: IgG1
• Host: Mouse
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
• Purification: Affinity purified by Protein G.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: PBS

Target Info

• Gene Symbol: TARDBP
• Gene Full Name: TAR DNA binding protein
• Alias Symbols: ALS10, TDP-43
• NCBI Gene Id: 23435
• Protein Name: TAR DNA-binding protein 43
• Description of Target: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
• Uniprot ID: Q13148
• Protein Accession #: NP_031401.1
• Nucleotide Accession #: NM_007375.3