TAP1 Recombinant Protein (OPCD00630)

Catalog No: OPCD00630

TAP1 Recombinant Protein (OPCD00630) using SDS-PAGE

TAP1 Recombinant Protein (OPCD00630) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Tyr525-Glu808
  Subcellular Location: Secreted.
  Isoelectric Point: 5.5
  Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:
  1. Splice Variants: Alternative splicing may create different sized proteins from the same gene.
  2. Relative charge: The composition of amino acids may affect the charge of the protein.
  3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
  4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
  5. Polymerization of the target protein: Dimerization, multimerization etc.
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 350 ug/mL (prior to lyoph)
• Purity: >90%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: TAP1
• Gene Full Name: transporter 1, ATP binding cassette subfamily B member
• Alias Symbols: ABC transporter, MHC 1;ABC17;ABCB2;antigen peptide transporter 1;APT1;ATP-binding cassette sub-family B member 2;ATP-binding cassette, sub-family B (MDR/TAP), member 2;D6S114E;peptide supply factor 1;peptide transporter involved in antigen processing 1;peptide transporter PSF1;peptide transporter TAP1;PSF1;PSF-1;really interesting new gene 4 protein;RING4;TAP1*0102N;TAP1N;transporter 1 ATP-binding cassette sub-family B;transporter 1, ATP-binding cassette, sub-family B (MDR/TAP);transporter associated with antigen processing;transporter, ATP-binding cassette, major histocompatibility complex, 1.
• NCBI Gene Id: 6890
• Protein Name: Antigen peptide transporter 1
• Description of Target: Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
• Uniprot ID: Q03518
• Protein Accession #: NP_000584.2
• Nucleotide Accession #: NM_000593.5
• Protein Size (# AA): Tyr525~Glu808
• Molecular Weight: 40kDa