TAP1 Antibody (OACD00942)

Catalog No: OACD00942

Western Blot with Human Recombinant Antigen peptide transporter 1

DAB staining on formalin fixed paraffin-embedded kidney tissue

Western Blot with Human Recombinant Antigen peptide transporter 1

DAB staining on formalin fixed paraffin-embedded kidney tissue

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Range: Tyr525 - Glu808
• Purification: Affinity Purified; Protein A Purified
• Concentration: 0.5 mg/ml
• Preservative: 0.02% NaN3
• Specificity: The antibody is a rabbit polyclonal antibody raised against TAP1. It has been selected for its ability to recognize TAP1 in immunohistochemical staining and western blotting.
• Formulation: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
• Application Data: Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
  Optimal working dilutions must be determined by end user.

Target Info

• Gene Symbol: TAP1
• Gene Full Name: transporter 1, ATP binding cassette subfamily B member
• Alias Symbols: ABC transporter, MHC 1;ABC17;ABCB2;antigen peptide transporter 1;APT1;ATP-binding cassette sub-family B member 2;ATP-binding cassette, sub-family B (MDR/TAP), member 2;D6S114E;peptide supply factor 1;peptide transporter involved in antigen processing 1;peptide transporter PSF1;peptide transporter TAP1;PSF1;PSF-1;really interesting new gene 4 protein;RING4;TAP1*0102N;TAP1N;transporter 1 ATP-binding cassette sub-family B;transporter 1, ATP-binding cassette, sub-family B (MDR/TAP);transporter associated with antigen processing;transporter, ATP-binding cassette, major histocompatibility complex, 1.
• NCBI Gene Id: 6890
• Protein Name: Antigen peptide transporter 1
• Description of Target: Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
• Uniprot ID: Q03518
• Protein Accession #: NP_000584.2
• Nucleotide Accession #: NM_000593
• Molecular Weight: 87 kDa