TANK Antibody (OAPB00434)

Catalog No: OAPB00434

Size:100UG

Western blot analysis of TANK in Daudi cell lysate with TANK antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry of TANK in Daudi cells with TANK antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. TANK Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: TANK antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: TANK antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TANK. The immunogen is located within amino acids 60 - 110 of TANK.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-TANK (OAPB00434) antibody is Catalog # OEPB02535
• Application Info: TANK antibody can be used for the detection of TANK by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user)
• Other Applications Image 1 Data: Immunocytochemistry of TANK in Daudi cells with TANK antibody at 2.5 ug/mL.
• Reference: Cheng G and Baltimore D. TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-kappaB activation. Genes Dev. 1996; 10:963-73.; Rothe M, Xiong J, Shu HB, et al. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Natl. Acad. Sci. USA 1996; 93:8241-6.;Pomerantz JL and Baltimore D. NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J. 1999; 18:6694-704.;Chariot A, Leonardi A, Muller J, et al. Association of the adaptor TANK with the IkappaB kinase (IKK) regulator NEMO connects IKK complexes with the IKKepsilon and TBK1 kinases. J. Biol. Chem.2002; 277:37029-36

Target Info

• Gene Symbol: TANK
• Gene Full Name: TRAF family member associated NFKB activator
• Alias Symbols: ITRAF;I-TRAF;TRAF family member-associated NF-kappa-B activator;TRAF2;TRAF-interacting protein.
• NCBI Gene Id: 10010
• Protein Name: TRAF family member-associated NF-kappa-B activator|TRAF family member-associated NF-kappa-B activator isoform a [Homo sapiens]
• Description of Target: Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage (PubMed:25861989). Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989). Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage (PubMed:25861989). May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2.
• Uniprot ID: Q92844
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004171
• Nucleotide Accession #: NM_001199135.1; NM_004180.2; NM_133484.1