Catalog No: OCOA10446
Size:20UG
Price: $179.00
SKU
OCOA10446
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TAF9B Protein Lysate (OCOA10446)

Datasheets/ManualsPrintable datasheet for TAF9B Protein Lysate (OCOA10446)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionTAF9B HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolTAF9B
Gene Full NameTATA-box binding protein associated factor 9b
Alias SymbolsDN7;DN-7;neuronal cell death-related protein 7;TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa;TAF9L;TAF9-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa;TAFII31L;TBP-associated factor 9L;TFIID-31;transcription initiation factor IID, 31kD subunit;transcription initiation factor TFIID subunit 9B;Transcription initiation factor TFIID subunit 9-like;transcription-associated factor TAFII31L.
NCBI Gene Id51616
Protein NameNM_015975, NP_057059, Q9HBM6
Description of TargetHomo sapiens TATA-box binding protein associated factor 9b (TAF9B), mRNA, Transcription initiation factor TFIID subunit 9B, transcription initiation factor TFIID subunit 9B [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q9HBM6
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_057059
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_015975
Molecular Weight27.4 kDa
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