Catalog No: OKCD01823 (Formerly GWB-KBBUZ4)
Size:96WELLS
Price: $675.00
SKU
OKCD01823
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for TAF13 ELISA Kit (Human) (OKCD01823) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TATA Box Binding Protein Associated Factor 13 (TAF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TATA Box Binding Protein Associated Factor 13 (TAF13) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TATA Box Binding Protein Associated Factor 13 (TAF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TATA Box Binding Protein Associated Factor 13 (TAF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.128 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of TATA Box Binding Protein Associated Factor 13 (TAF13). No significant cross-reactivity or interference between TATA Box Binding Protein Associated Factor 13 (TAF13) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | TAF13 |
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Gene Full Name | TATA-box binding protein associated factor 13 |
Alias Symbols | MRT60;TAF(II)18;TAF13 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 18kDa;TAF2K;TAFII18;TAFII-18;TATA box binding protein (TBP)-associated factor, RNA polymerase II, K, 18kD;transcription initiation factor TFIID 18 kD subunit;transcription initiation factor TFIID 18 kDa subunit;transcription initiation factor TFIID subunit 13. |
NCBI Gene Id | 6884 |
Protein Name | Transcription initiation factor TFIID subunit 13 |
Description of Target | Component of the DNA-binding general RNA polymerase II transcription factor IID complex (TFIID). TFIID plays a critical role in the regulation of gene transcription in eukaryotic cells. |
Uniprot ID | Q15543 |
Protein Accession # | NP_005636.1 |
Nucleotide Accession # | NM_005645.3. |
Protein Size (# AA) | 124 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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