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Catalog No: OKCD01823 (Formerly GWB-KBBUZ4)
Size:96WELLS
Price: $675.00
SKU
OKCD01823
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Datasheets/ManualsPrintable datasheet for TAF13 ELISA Kit (Human) (OKCD01823)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TATA Box Binding Protein Associated Factor 13 (TAF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TATA Box Binding Protein Associated Factor 13 (TAF13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TATA Box Binding Protein Associated Factor 13 (TAF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TATA Box Binding Protein Associated Factor 13 (TAF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TAF13 Microplate96 Wells (12 x 8 Well Strips)
TAF13 Lyophilized Standard2 x 20 ng
100X Biotinylated TAF13 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.128 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of TATA Box Binding Protein Associated Factor 13 (TAF13). No significant cross-reactivity or interference between TATA Box Binding Protein Associated Factor 13 (TAF13) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTAF13
Gene Full NameTATA-box binding protein associated factor 13
Alias SymbolsMRT60;TAF(II)18;TAF13 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 18kDa;TAF2K;TAFII18;TAFII-18;TATA box binding protein (TBP)-associated factor, RNA polymerase II, K, 18kD;transcription initiation factor TFIID 18 kD subunit;transcription initiation factor TFIID 18 kDa subunit;transcription initiation factor TFIID subunit 13.
NCBI Gene Id6884
Protein NameTranscription initiation factor TFIID subunit 13
Description of TargetComponent of the DNA-binding general RNA polymerase II transcription factor IID complex (TFIID). TFIID plays a critical role in the regulation of gene transcription in eukaryotic cells.
Uniprot IDQ15543
Protein Accession #NP_005636.1
Nucleotide Accession #NM_005645.3.
Protein Size (# AA)124
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