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Catalog No: OKEH03314
Size:96T
Price: $525.00
SKU
OKEH03314
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TAC3 ELISA Kit (Rat) (OKEH03314) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Tac3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tac3 and incubated. The Tac3 found in the sample or standards competes with the biotinylated Tac3 for limited binding sites on the immobilized anti-Rat Tac3 antibody. Excess unbound biotinylated Tac3 and sample or standard Tac3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tac3 captured in the well and inversely proportional to the amount of Tac3 which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Tac3 Microplate96 Wells (12 x 8 Well strips)
Tac3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Tac3-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity41.1 pg/mL
SpecificityNatural and recombinant Rat Tachykinin-3
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolTac3
Gene Full Nametachykinin precursor 3
Alias Symbolsneurokinin B;Tac2;tachykinin 2;tachykinin 3;tachykinin-3.
NCBI Gene Id29191
Protein NameP08435
Description of TargetTachykinin-3
Uniprot IDhttps://www.uniprot.org/uniprot/P08435
Protein Accession #NP_062035.1
Nucleotide Accession #NM_019162.2
Protein Size (# AA)116
Molecular Weight13 kDa
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