T.gondii p30 Protein (OPPA01481)

Catalog No: OPPA01481 (Formerly GWB-6EB9ED)

Size:100UG

The life cycle of Toxoplasma gondii has two phases. The coccidia like takes place only in members of the Felidae family which makes these animals the parasite's primary host. The asexual part of the life cycle can take place in any warm-blooded animal, like other mammals(including felines) and birds. T. gondii constructing daughter scaffolds within the mother cell. In the intermediate hosts (including felines), the parasite invades cells, forming intracellular so-called parasitophorous vacuoles containing bradyzoites, the slowly replicating form of the parasit. Vacuoles form tissue cysts mainly within the muscles and brain. Since they are within cells, the host's immune system does not detect these cysts. Resistance to antibiotics varies, but the cysts are very difficult to eradicate entirely. Within these vacuoles T. gondii propagates by a series of binary fissions until the infected cell eventually bursts and tachyzoites are released. Tachyzoites are the motile, asexually reproducing form of the parasite. Unlike the bradyzoites, the free tachyzoites are usually efficiently cleared by the host's immune response, although some manage to infect cells and form bradyzoites, thus maintaining the infection.

Product Info

• Predicted Species Reactivity: Toxoplasma gondii
• Product Format: Liquid 50mM Tris-HCl (pH 8), 1.5M urea and 50% glycerol.
• Application: ELISA, WB
• Reconstitution and Storage: T.gondii, although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze/thaw cycles.
• Purification: Purified by proprietary chromatographic technique.
• Concentration: Varies by lot. See vial for concentration.
• Purity: Toxoplasma protein is >95% pure as determined by 10% PAGE (coomassie staining).
• Specificity: Immunoreactive with sera of Toxoplasma gondii-infected individuals.
• Application Info: Toxoplasma antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma gondii- with minimal specificity problems.

Target Info

• Gene Symbol: Toxoplasma P30
• Alias Symbols: T.gondii p30, Toxoplasma Gondii p30 (SAG1) Recombinant
• Description of Target: Recombinant Toxoplasma Gondii P30 (SAG 1)
• Protein Size (# AA): Recombinant
• Molecular Weight: The E.Coli derived recombinant protein contains the p30 (SAG1) immunodominant regions, amino acids 45-198 and fused to a 26kDa GST tag, having a total Mw of 42.3 kda.