SYT17 ELISA Kit (Mouse) (OKWB00242)

Catalog No: OKWB00242

Size:96T

Mouse SYT17 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-105%	89-101%	97-105%	87-100%
  EDTA Plasma (n=5)	84-101%	84-101%	82-97%	82-94%
  Heparin Plasma (n=5)	82-99%	91-98%	83-94%	80-92%

• ELISA Kit Principle: Aviva Systems Biology Syt17 ELISA Kit (Mouse) (OKWB00242) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with Syt17. Sample or standards are added to the wells along with a Syt17 biotinylated detector antibody and incubated. The Syt17 found in the sample or standards inhibits the Syt17 detector antibody from binding to the immobilized Syt17 on the plate. Excess unbound sample or standard Syt17 and detector antibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of Syt17 biotinylated detector antibody bound in the well and inversely proportional to the amount of Syt17 which was contained in the sample or standard.
• ELISA Kit Range: 7.813-500pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	90-104	98
  EDTA Plasma (n=5)	85-101	93
  Heparin Plasma (n=5)	87-105	98

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Syt17 Microplate: 96 Wells (12 x 8 Well strips)
  • Syt17 Lyophilized Standard: 2 x 100 ng
  • 100X Syt17 Biotinylated Detector Anitbody: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.938 ng/mL
• Assay Info: Assay Type: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: Kng1
• Gene Full Name: kininogen 1
• Alias Symbols: H-kin;H-kininigen;kininogen-1;Kng;L-ki;L-kininogen.
• NCBI Gene Id: 16644
• Protein Name: Kininogen-1
• Description of Target: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting (By similarity).
• Uniprot ID: O08677
• Protein Accession #: NP_619590
• Nucleotide Accession #: NM_138649