SYNCRIP Antibody (OAAB18303)

Catalog No: OAAB18303

Size:100ul

Western blot analysis of lysates from HT-29, Hela cell line (from left to right), using hnRNP Q Antibody (C10139).
C10139 was diluted at 1:1000 at each lane.
A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Lysates at 35ug per lane.
Dilution: 1:1000

Immunofluorescence analysis of HepG2 cells, using hnRNP Q antibody.
Dilution: 1:100

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IF
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Application Info: WB: 1:1000
  IF: 1:100
• Reference: Harris C.E., et al.J. Virol. 73:72-80(1999).
  Du G., et al.Chin. Sci. Bull. 45:343-349(2000).
  Mourelatos Z., et al.EMBO J. 20:5443-5452(2001).
  Suzuki Y., et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
  Mungall A.J., et al.Nature 425:805-811(2003).

Target Info

• Gene Symbol: SYNCRIP
• Alias Symbols: PP68, NSAP1, GRYRBP, HNRNPQ, HNRPQ1, GRY-RBP, hnRNP-Q
• NCBI Gene Id: 10492
• Description of Target: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD- mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma- induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; seems not to be essential for GAIT complex function.
• Uniprot ID: O60506
• Molecular Weight: 70 kDa