Synaptotagmin-7 Antibody (OASE00272)

Catalog No: OASE00272

Size:100ug

Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug).. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Brevican Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide
• Clonality: Monoclonal
• Clone: S275-14
• Isotype: IgG2B
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF
  Certificate of Analysis:1 ug/mL was sufficient for detection of Synaptotagmin-7 in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  Cellular Localization: Cytoplasmic vesicle
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Application Info: WB: 1:1000
  ICC/IF: 1:100
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Publications:

  CREB Promotes Beta Cell Gene Expression by Targeting Its Coactivators to Tissue-Specific Enhancers. Mol Cell Biol. 39, (2019). 31182641

• Reference: 1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815.
  2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
  
• Description:
  View product citations for Synaptotagmin-7 Antibody (OASE00272) on CiteAb

Target Info

• Gene Symbol: Synaptotagmin 7
• Gene Full Name: synaptotagmin VII
• Alias Symbols: IPCA-7, PCANAP7, Prostate cancer-associated protein 7, SYT-7, SYT7, Synaptotagmin 7, Synaptotagmin VII, SytVII
• NCBI Gene Id: 54525
• Description of Target: Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
• Uniprot ID: Q9R0N7
• Protein Accession #: NP_061271.1
• Molecular Weight: 45 kDa