SYK Antibody (Phospho-Tyr525/Tyr526) (OABF01244)

Catalog No: OABF01244

Size:100UL

L1 mouse embryo lysates L2 mouse thymus lysates probed with Anti Phospho-Syk (Tyr525/526) Polyclonal Antibody, Unconjugated (OABF01244) at 1:200 overnight at 4C. Followed by conjugation to secondary antibody at 1:3000 for 90 min at 37C. Predicted band 70kD. Observed band size:70kD.

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Phospho-Syk (Tyr525/526) Polyclonal Antibody, Unconjugated (OABF01244) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Dog|Guinea Pig|Human|Mouse|Porcine|Rabbit|Rat
• Product Format: Liquid Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
  Modification Site: Tyr525 + Tyr526
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic phosphopeptide derived from human Syk around the phosphorylation site of Tyr525/526
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Application Info: WB: 1:100~1000
  IHC-P: 1:100~500
  Fluorescent-IHC: 1:50~200

Target Info

• Gene Symbol: SYK
• Gene Full Name: spleen associated tyrosine kinase
• Alias Symbols: IMD82;p72-Syk;spleen tyrosine kinase;tyrosine-protein kinase SYK.
• NCBI Gene Id: 6850
• Protein Name: Tyrosine-protein kinase SYK
• Description of Target: Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response (By similarity).
• Uniprot ID: P43405