Catalog No: OKEH01710
Size:96T
Price: $525.00
SKU
OKEH01710
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SWAP70 ELISA Kit (Human) (OKEH01710) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SWAP70 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SWAP70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SWAP70 captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
SWAP70 Microplate96 Wells (12 x 8 Well strips)
SWAP70 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SWAP70 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.082 ng/mL
SpecificityNatural and recombinant Human Switch-associated protein 70
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSWAP70
Gene Full Nameswitching B cell complex subunit SWAP70
Alias SymbolsHSPC321;SWAP switching B-cell complex 70kDa subunit;SWAP switching B-cell complex subunit 70;SWAP-70;switch-associated protein 70.
NCBI Gene Id23075
Protein NameQ9UH65
Description of TargetSwitch-associated protein 70
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UH65
Protein Accession #NP_001284643.1
Nucleotide Accession #NM_001297714.1
Protein Size (# AA)585
Molecular Weight68 kDa
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