SWAP70 Antibody - middle region (OAAB10718)

Catalog No: OAAB10718

Size:400ul

Western blot analysis of SWAP70 (arrow) using rabbit polyclonal SWAP70 Antibody (Center) (Cat# OAAB10718). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SWAP70 gene (Lane 2) (Origene Technologies).

Western blot analysis of anti-SWAP70 Antibody (Center) (Cat# OAAB10718) in HepG2 cell line lysates (35ug/lane). SWAP70(arrow) was detected using the purified polyclonal antibody.

Product Info

• Predicted Species Reactivity: Human, Mouse, Cow
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Precautions: SWAP70 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity)
  Cellular Location: Cytoplasm. Cell membrane. Nucleus. Cell projection, lamellipodium. Note=In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamell
  Tissue Location: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus- associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SWAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-256 amino acids from the Central region of human SWAP70.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: SWAP70
• Alias Symbols: HSPC321, SWAP-70
• NCBI Gene Id: 23075
• Protein Name: Switch-associated protein 70
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9UH65
• Protein Accession #: NP_055870.2
• Molecular Weight: 68998 Da