SV2A Antibody (OAEB02116)

Catalog No: OAEB02116 (Formerly GWB-4EADCD)

Size:100UG

OAEB02116 (0.3ug/ml) staining of rat brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02116 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

OAEB02116 Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, IF
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SV2A
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%
• Peptide Sequence: C-TVELYPSDKRTTA
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx 80kDa band observed in rat brain lysates (calculated MW of 82.6kDa according to NP_476558.1 (rat)). Recommended concentration: 0.3-1ug/ml.
  An additonal band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide.

Target Info

• Reference: * Dong M, Yeh F, Tepp WH, Dean C, Johnson EA, Janz R, Chapman ER.; SV2 is the protein receptor for botulinum neurotoxin A.; Science. 2006 Apr 28;312(5773):592-6. Epub 2006 Mar 16.; Pubmed 16543415
• Gene Symbol: Sv2a|SV2A
• Gene Full Name: synaptic vesicle glycoprotein 2 a|synaptic vesicle glycoprotein 2a|synaptic vesicle glycoprotein 2A
• Alias Symbols: AI746429;calcium regulator SV2A;mKIAA0736;SV2;synaptic vesicle glycoprotein 2A;synaptic vesicle protein 2;synaptic vesicle protein 2A;synaptic vesicle transmembrane transporter (SV2) RNA.
• NCBI Gene Id: 117559|64051|9900
• Protein Name: Synaptic vesicle glycoprotein 2A
• Uniprot ID: Q7L0J3
• Protein Accession #: NP_055664.2
• Molecular Weight: 82.7; 20.6