SUZ12 ELISA Kit (Human) (OKCD02070)

Catalog No: OKCD02070 (Formerly GWB-KBBKB7)

Size:96WELLS

Human SUZ12 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressor Of Zeste 12 Homolog (SUZ12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppressor Of Zeste 12 Homolog (SUZ12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressor Of Zeste 12 Homolog (SUZ12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Suppressor Of Zeste 12 Homolog (SUZ12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppressor Of Zeste 12 Homolog (SUZ12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppressor Of Zeste 12 Homolog (SUZ12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SUZ12 Microplate: 96 Wells (12 x 8 Well Strips)
  • SUZ12 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated SUZ12 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Suppressor Of Zeste 12 Homolog (SUZ12). No significant cross-reactivity or interference between Suppressor Of Zeste 12 Homolog (SUZ12) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SUZ12
• Gene Full Name: SUZ12 polycomb repressive complex 2 subunit
• Alias Symbols: chET 9 protein;CHET9;chromatin precipitated E2F target 9 protein;IMMAS;JJAZ1;joined to JAZF1 protein;polycomb protein SUZ12;suppressor of zeste 12 protein homolog.
• NCBI Gene Id: 23512
• Protein Name: Polycomb protein SUZ12
• Description of Target: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
• Uniprot ID: Q15022
• Protein Accession #: NP_056170.2
• Nucleotide Accession #: NM_015355.3
• Protein Size (# AA): 739