SUV39H1 Antibody (OAAB06640)

Catalog No: OAAB06640

Size:400ul

Western analysis of extracts from 293 cells using SUV39H1 antibody.

SUV39H1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Clonality: Monoclonal
• Clone: 42AT239.96.72
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Additional Information: Precautions: SUV39H1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone- modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus
  Cellular Location: Nucleus. Chromosome, centromere. Note=Associates with centromeric constitutive heterochromatin
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SUV39H1 antibody was raised using purified recombinant GST fusion protein encoding N-terminal of human SUV39H1.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  SUV39H1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:100~500

Target Info

• Gene Symbol: SUV39H1
• Alias Symbols: MG44, KMT1A, SUV39H, H3-K9-HMTase 1
• NCBI Gene Id: 6839
• Protein Name: Histone-lysine N-methyltransferase SUV39H1
• Description of Target: Purified Mouse Monoclonal Antibody (Mab)
• Uniprot ID: O43463
• Protein Accession #: NP_003164.1
• Molecular Weight: 47907 Da