SUMO2 ELISA Kit (Human) (OKCD01900)

Catalog No: OKCD01900 (Formerly GWB-KBBHL8)

Size:96WELLS

Human SUMO2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 2 (SUMO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Small Ubiquitin Related Modifier Protein 2 (SUMO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 2 (SUMO2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 2 (SUMO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SUMO2 Microplate: 96 Wells (12 x 8 Well Strips)
  • SUMO2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated SUMO2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.126 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Small Ubiquitin Related Modifier Protein 2 (SUMO2). No significant cross-reactivity or interference between Small Ubiquitin Related Modifier Protein 2 (SUMO2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SUMO2
• Gene Full Name: small ubiquitin like modifier 2
• Alias Symbols: HSMT3;sentrin 2;Sentrin-2;small ubiquitin-related modifier 2;SMT3 homolog 2;SMT3 suppressor of mif two 3 homolog 2;Smt3A;SMT3B;SMT3H2;SUMO3;SUMO-3;ubiquitin-like protein SMT3A;ubiquitin-like protein SMT3B.
• NCBI Gene Id: 6613
• Protein Name: Small ubiquitin-related modifier 2
• Description of Target: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744).
• Uniprot ID: P61956
• Protein Accession #: NP_001005849.1
• Nucleotide Accession #: NM_001005849.1
• Protein Size (# AA): 95