Catalog No: OKCD00356 (Formerly GWB-KBBKM9)
Size:96WELLS
Price: $675.00
SKU
OKCD00356
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Datasheets/ManualsPrintable datasheet for SUMF1 ELISA Kit (Human) (OKCD00356)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfatase Modifying Factor 1 (SUMF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sulfatase Modifying Factor 1 (SUMF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sulfatase Modifying Factor 1 (SUMF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sulfatase Modifying Factor 1 (SUMF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfatase Modifying Factor 1 (SUMF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfatase Modifying Factor 1 (SUMF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SUMF1 Microplate96 Wells (12 x 8 Well Strips)
SUMF1 Lyophilized Standard2 x 10 ng
100X Biotinylated SUMF1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.064 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sulfatase Modifying Factor 1 (SUMF1). No significant cross-reactivity or interference between Sulfatase Modifying Factor 1 (SUMF1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSUMF1
Gene Full Namesulfatase modifying factor 1
Alias SymbolsAAPA3037;C-alpha-formylglycine-generating enzyme 1;FGE;FGly-generating enzyme;formylglycine-generating enzyme;Sulfatase-modifying factor 1;UNQ3037.
NCBI Gene Id285362
Protein NameFormylglycine-generating enzyme
Description of TargetOxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent (PubMed:12757706, PubMed:15657036, PubMed:15907468, PubMed:25931126, PubMed:16368756, PubMed:21224894). 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile (PubMed:12757706, PubMed:15657036, PubMed:15907468, PubMed:25931126, PubMed:16368756). Known substrates include GALNS, ARSA, STS and ARSE (PubMed:12757706, PubMed:15907468, PubMed:15657036).
Uniprot IDQ8NBK3
Protein Accession #NP_001158146
Nucleotide Accession #NM_001164674.1
Protein Size (# AA)374
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