Catalog No: OCOA04800
Size:20UG
Price: $179.00
SKU
OCOA04800
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SULT1A1 Protein Lysate (OCOA04800)

Datasheets/ManualsPrintable datasheet for SULT1A1 Protein Lysate (OCOA04800)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSULT1A1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSULT1A1
Gene Full Namesulfotransferase family 1A member 1
Alias Symbolsaryl sulfotransferase 1;HAST1/HAST2;Phenol sulfotransferase 1;phenol-sulfating phenol sulfotransferase 1;P-PST;P-PST 1;PST;ST1A1;ST1A3;STP;STP1;sulfotransferase 1A1;sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1;Thermostable phenol sulfotransferase;thermostable phenol sulfotransferase1;ts-PST;TSPST1.
NCBI Gene Id6817
Protein NameNM_177529, NP_803565, P50225
Description of TargetHomo sapiens sulfotransferase family 1A member 1 (SULT1A1), transcript variant 2, mRNA, Sulfotransferase 1A1, sulfotransferase 1A1 isoform a [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P50225
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_803565
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_177529
Molecular Weight34 kDa
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