SULT1A1 ELISA Kit (Rat) (OKCD02792)

Catalog No: OKCD02792

Size:96WELLS

Rat SULT1A1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfotransferase 1A1 (SULT1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sulfotransferase 1A1 (SULT1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sulfotransferase 1A1 (SULT1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sulfotransferase 1A1 (SULT1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SULT1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • SULT1A1 Lyophilized Standard: 2
  • 100X Biotinylated SULT1A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phenol Sulfotransferase (PST). No significant cross-reactivity or interference between Phenol Sulfotransferase (PST) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Sult1a1
• Gene Full Name: sulfotransferase family 1A member 1
• Alias Symbols: Aryl sulfotransferase;Aryl sulfotransferase cytosolic, 1A, phenol-preferring, member 3;aryl sulfotransferase IV;ASTIV;minoxidil sulfotransferase;Mx-ST;Phenol sulfotransferase;Phenol sulfotransferase 1a1;PST-1;St1a1;Stm;Stp1;sulfokinase;sulfotransferase 1A1;sulfotransferase family 1A, phenol-preferring, member 1;sulfotransferase family, cytosolic, 1A, phenol preferring;sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1;sulphotransferase family cytosolic 1A phenol-prefering member 1;Sult1a3;tyrosine-ester sulfotransferase.
• NCBI Gene Id: 83783
• Protein Name: Sulfotransferase 1A1
• Description of Target: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.
• Uniprot ID: P17988
• Protein Accession #: NP_114022.1
• Nucleotide Accession #: NM_031834.1
• Protein Size (# AA): 291