Catalog No: OKEH08060
Size:96T
Price: $875.00
SKU
OKEH08060
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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SULT1A1 ELISA Kit (Dog) (OKEH08060)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)84-93% (n = 20)93-102% (n = 20)80-89% (n = 20)108-118% (n = 20)
EDTA plasma(n=5)90-102% (n = 20)100-110% (n = 20)84-93% (n = 20)103-115% (n = 20)
heparin plasma(n=5)105-114% (n = 20)111-121% (n = 20)105-105% (n = 20)113-123% (n = 20)
ELISA Kit PrincipleAviva Systems Biology SULT1A1 ELISA Kit (Dog) (OKEH08060) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SULT1A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SULT1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SULT1A1 captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=8.3% (n = 20)
Mean Inter-assay CV%: <=8.4% (n = 20)
ELISA Kit Component
ComponentAmount
SULT1A1 Microplate96 Wells (12 x 8 Well strips)
SULT1A1 Lyophilized Standard2
100X Biotinylated SULT1A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Catalyzes the sulfate conjugation of catecholamines and phenolic drugs and neurotransmitters. Acts on p-nitrophenol (PNP), alpha-naphthol, dopamine, minoxidil and beta-estradiol but not dehydroepiandrosterone.
::Subcellular Location: Cytoplasm
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Dog Sulfotransferase 1A1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSULT1A1
Gene Full Namesulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
Alias Symbolsaryl sulfotransferase;cSULT1A1;phenol sulfotransferase;phenol-sulfating phenol sulfotransferase;P-PST;PST;ST1A1;sulfotransferase 1A1.
NCBI Gene Id403892
Protein NameQ29476
Description of TargetSulfotransferase 1A1
Uniprot IDhttps://www.uniprot.org/uniprot/Q29476
Protein Accession #NP_001003223.1
Nucleotide Accession #NM_001003223.1
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