SUCLA2 ELISA Kit (Human) (OKEH08613)

Catalog No: OKEH08613

Size:96T

Human SUCLA2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	103-103% (n = 20)	86-96% (n = 20)	106-118% (n = 20)	90-100% (n = 20)
  EDTA plasma(n=5)	99-109% (n = 20)	82-91% (n = 20)	108-116% (n = 20)	98-107% (n = 20)
  heparin plasma(n=5)	104-113% (n = 20)	104-112% (n = 20)	97-107% (n = 20)	110-119% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology SUCLA2 ELISA Kit (Human) (OKEH08613) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SUCLA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SUCLA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SUCLA2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.0% (n = 20)
  Mean Inter-assay CV%: <=7.3% (n = 20)
• ELISA Kit Component:
  • SUCLA2 Microplate: 96 Wells (12 x 8 Well strips)
  • SUCLA2 Lyophilized Standard: 2
  • 100X Biotinylated SUCLA2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA (PubMed:15877282). The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
  Subcellular Location: Mitochondrion
  Unigene: Hs.743361
  SMR: Q9P2R7
  String: 9606.ENSP00000367923
  KEGG: hsa:8803
  MIM: 603921
  Pfam: PF08442
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.032ng/mL
• Specificity: Natural and recombinant Human Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SUCLA2
• Gene Full Name: succinate-CoA ligase ADP-forming subunit beta
• Alias Symbols: A-BETA;A-SCS;ATP-specific succinyl-CoA synthetase subunit beta;ATP-specific succinyl-CoA synthetase, beta subunit;LINC00444;long intergenic non-protein coding RNA 444;mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta;MTDPS5;renal carcinoma antigen NY-REN-39;SCS-betaA;succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial;succinate-CoA ligase ADP-forming beta subunit;succinate-CoA ligase beta subunit;succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial;succinyl-CoA synthetase beta-A chain.
• NCBI Gene Id: 8803
• Protein Name: Q9P2R7
• Description of Target: Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q9P2R7
• Protein Accession #: NP_003841.1
• Nucleotide Accession #: NM_003850.2