SUCLA2 Antibody (OAGA00790)

Catalog No: OAGA00790

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
B: Molt-4
10% SDS PAGE
OAGA00790 diluted at 1:1000

Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
OAGA00790 diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SUCLA2(OAGA00790) antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed A431, using SUCLA2(OAGA00790) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain
  Cellular Localization: Mitochondrion
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 138 and 416 of SUCLA2 (Uniprot ID#Q9P2R7)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: SUCLA2
• Gene Full Name: Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
• Alias Symbols: A-SCS, A-BETA, MTDPS5, LINC00444, SCS-betaA
• NCBI Gene Id: 8803
• Protein Name: Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
• Uniprot ID: Q9P2R7
• Protein Accession #: NP_003841.1
• Molecular Weight: 50 kDa