STUB1 ELISA Kit (Mouse) (OKCD09277)

Catalog No: OKCD09277

Size:96WELLS

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to STIP1 Homology And U-Box Containing Protein 1 (STUB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to STIP1 Homology And U-Box Containing Protein 1 (STUB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STIP1 Homology And U-Box Containing Protein 1 (STUB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of STIP1 Homology And U-Box Containing Protein 1 (STUB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level STIP1 Homology And U-Box Containing Protein 1 (STUB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level STIP1 Homology And U-Box Containing Protein 1 (STUB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • STUB1 Microplate: 96 Wells (12 x 8 Well strips)
  • STUB1 Lyophilized Standard: 2
  • 100X STUB1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: STUB1
• Gene Full Name: STIP1 homology and U-Box containing protein 1
• Alias Symbols: CH, Chip, AW046544, 0610033N24Rik, 2210017D18Rik, 2310040B03Rik
• NCBI Gene Id: 56424
• Protein Name: STIP1 homology and U box-containing protein 1
• Description of Target: E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. collaborates with atxn3 in the degradation of misfolded chaperone substrates: atxn3 restricting the length of ubiquitin chain attached to stub1/chip substrates and preventing further chain extension. ubiquitinates nos1 in concert with hsp70 and hsp40. modulates the activity of several chaperone complexes, including hsp70, hsc70 and hsp90. mediates transfer of non-canonical short ubiquitin chains to hspa8 that have no effect on hspa8 degradation. mediates polyubiquitination of dna polymerase beta (polb) at 'lys-41', 'lys-61' and 'lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the huwe1/arf-bp1-dependent monoubiquitination and leading to polb-degradation by the proteasome. mediates polyubiquitination of cyp3a4. ubiquitinates epha2 and may regulate the receptor stability and activity through proteasomal degradation. negatively regulates the suppressive function of regulatory t-cells (treg) during inflammation by mediating the ubiquitination and degradation of foxp3 in a hspa1a/b-dependent manner (pubmed:23973223).
• Uniprot ID: Q9WUD1
• Protein Accession #: NP_062693.1
• Nucleotide Accession #: NM_019719.3