Streptomycin ELISA Kit (OKAO00125)

Catalog No: OKAO00125

Size:96 Wells

General Streptomycin ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: General
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Streptomycin ELISA Kit (OKAO00125) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Streptomycin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Streptomycin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
• ELISA Kit Range: 0.1-8.1 ppb
• ELISA Kit Recovery:

  Sample	Amount
  Liver(Chicken)	85% +/- 10%
  Milk	95% +/- 15%
  Honey, Royal jelly	75% +/- 15%

• ELISA Kit Reproducibility: Mean Intra-Assay: CV<8% (n= 3 x 20)
  Mean Inter-Assay: CV<12% (n= 3 x 40)
• ELISA Kit Component:
  • Streptomycin Microplate: 96 Wells (12 x 8 Well Strips)
  • Streptomycin Standards: 6 x 1 mL
  • Streptomycin Rabbit Detector Antibody: 1 x 7 mL
  • Anti-Rabbit / HRP Conjugate: 1 x 12 mL
  • Detection Reagent A: 1 x 7 mL
  • Detection Reagent B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
  • 20X Wash Buffer: 2 x 20 mL
  • 5X Sample Prep Buffer: 3 x 15 mL
• Additional Information: CAS: 57-92-1
  PubChem: 19649
  Molecular Formula: C21H39N7O12
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Liver (Chicken), milk, honey, royal jelly.
• Sensitivity:

  Sample	Amount
  Liver(Chicken)	1 ppb
  Milk	1 ppb
  Honey	1 ppb
  Royal jelly	1 ppb

• Specificity:

  Substance	Cross Reactivity Rate
  Streptomycin	100%
  Dihydrostreptomycin	100%
  Kalamycin	<1%
  Gentamycin	<1%

• Assay Info: Assay Methodology: Competitive Inhibition ELISA

Target Info

• Alias Symbols: SM, Streptomycin, Streptomycin A, Agrimycin, Strepcen, Agrept, Gerox
• Protein Name: Streptomycin
• Description of Target: eptomycin is an Aminoglycoside Antibacterial and Antimycobacterial. The chemical classification of streptomycin is Aminoglycosides.
• Molecular Weight: 581.57406 g/mol