Catalog No: OPPA01253 (Formerly GWB-43A5FD)
Size:20ug
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Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for OPPA01253 |
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Product Format | Liquid 10mM K2HLPO4-KH2PO4 (pH 7.3) |
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Host | E. Coli |
Application | IA, WB |
Reconstitution and Storage | Streptavidin-NC although stable at 4°C for 3 weeks, should be stored below -18°C. Please prevent freeze thaw cycles. |
Concentration | 3.24 mg/ml |
Purity | Greater than 93.0% as determined by SDS-PAGE |
Application Info | Calibrators and controls for immunoassays and western blot standards. |
Gene Symbol | Streptavidin-NC |
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Alias Symbols | Streptavidin-NC, Streptavidin-NC Recombinant |
Description of Target | Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH. |
Molecular Weight | 24 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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