STREPTAVIDIN Fluorescein Conjugated (OORA00136)

Catalog No: OORA00136

Size:1MG

Product Info

• Predicted Species Reactivity: Streptavidin
• Product Format: Lyophilized 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 10 mg/ml BSA (Immunoglobulin & Protease free) with 0.01% (w/v) sodium azide
• Conjugation: FITC
• Application: Dot blot, Flow cytometry, Fluorescence-linked immunosorbent assay, Immunofluorescence
  Excitation/Emission Wavelengths: 495-528nm
  F/P Ratio: 2.2 moles FITC per mole of
  Conjugation Chemistry: Isothiocyanate
• Reconstitution and Storage: Restore with 1.0 ml deionized water (or equivalent). Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration is one (1) year from date of receipt.
• Concentration: 1 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Streptavidin.
• Application Info: IF: 1:1000~5000
  FLISA: 1:10000~50000.
• Purification: This product was prepared from chromatographically pure Streptavidin.

Target Info

• Alias Symbols: FITC
• Description of Target: Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Rockland conjugates a broad group of secondary antibodies to many of the classic fluorescent markers including fluorescein, rhodamine, Texas Red, and Phycoerythrin (RPE). All of the conjugates are ideal for various immunofluorescence based assays including fluorescent western blotting, immunofluorescence microscopy, FLISA, and more. Rockland also produces many next generation fluorochrome dyes designed for detection of primary antibodies in multiplex, multi-color analysis.
• Molecular Weight: 390