Stratifin(SFN) Polyclonal Antibody (OABB00748)

Catalog No: OABB00748

Size:100UG

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Lane 1: Hela Cell Lysate
Lane 2: A549 Cell Lysate
Lane3: A549 Cell Lysate
Lane4:Colo320 Cell Lysate
Lane5: SE620 Cell Lysate

IHC(P):Human Esophageal squamous cell carcinoma Cancer Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
  Background: Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al. (1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunocytochemistry : 0.5-1 ug/ml: Human
  Immunocytochemistry/Immunofluorescence: 2 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993.
  2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002.
  3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for 14-3-3 protein sigma(SFN) detection. Tested with WB, IHC-P, ICC, ICC/IF, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: SFN
• Gene Full Name: stratifin
• Alias Symbols: 14-3-3 protein sigma;epithelial cell marker protein 1;Stratifin;YWHAS.
• NCBI Gene Id: 2810
• Protein Name: 14-3-3 protein sigma
• Description of Target: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
• Uniprot ID: P31947
• Molecular Weight: 27774 MW