STRAP Antibody (OACA07747)

Catalog No: OACA07747

Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human placenta tissue at dilution 1:100

Western blot
All lanes: STRAP antibody at 8ug/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: mouse brain tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 39,40 kDa
Observed band size: 38 kDa

Western blot
All lanes: STRAP antibody at 8ug/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: mouse brain tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 39,40 kDa
Observed band size: 38 kDa

Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human placenta tissue at dilution 1:100

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Serine-threonine kinase receptor-associated protein (1-350AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"Molecular cloning and characterization of human MAWD, a novel protein containing WD-40 repeats frequently overexpressed in breast cancer."Matsuda S., Katsumata R., Okuda T., Yamamoto T., Miyazaki K., Senga T., Machida K., Thant A.A., Nakatsugawa S., Hamaguchi M.Cancer Res. 60:13-17(2000). [2]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A. Genome Res. 11:422-435(2001).

Target Info

• Gene Symbol: STRAP
• Gene Full Name: serine/threonine kinase receptor associated protein
• Alias Symbols: MAP activator with WD repeats;MAPK activator with WD repeats;MAWD;mitogen-activated protein kinase activator with WD repeats;PT-WD;serine-threonine kinase receptor-associated protein;unr-interacting protein;UNRIP;WD-40 repeat protein PT-WD.
• NCBI Gene Id: 11171
• Protein Name: Serine-threonine kinase receptor-associated protein
• Description of Target: The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.
• Uniprot ID: Q9Y3F4
• Protein Accession #: NP_009109
• Nucleotide Accession #: NM_007178