Catalog No: OCOA11904
Size:20UG
Price: $275.00
SKU
OCOA11904
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STON1 Protein Lysate (OCOA11904)

Datasheets/ManualsPrintable datasheet for STON1 Protein Lysate (OCOA11904)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSTON1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSTON1, STON1-GTF2A1L
Gene Full NameSTON1-GTF2A1L readthrough, stonin 1
Alias SymbolsALF;General transcription factor II A, 1-like factor;GTF2A1L;GTF2A1LF;SALF;SBLF;STN1;STNB1;STON1-GTF2A1L protein;stoned B homolog 1;stoned B/TFIIA-alpha/beta-like factor;stoned B-like factor;stoned-b1;stonin-1;TFIIA-alpha and beta-like factor.
NCBI Gene Id11037, 286749
Protein NameB2RB25, NM_006873, NP_006864, Q9Y6Q2
Description of TargetcDNA, FLJ95267, highly similar to Homo sapiens stoned B-like factor (SBLF), mRNA, Homo sapiens stonin 1 (STON1), transcript variant 2, mRNA, Stonin-1, stonin-1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y6Q2
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_006864
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_006873
Molecular Weight83 kDa
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