STK4 Antibody (OAGA02136)

Catalog No: OAGA02136

Size:100 ul

STK4 antibody detects STK4 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STK4 antibody (OAGA02136) diluted by 1:1000.

STK4 antibody detects STK4 protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
STK4 antibody (OAGA02136) dilution: 1:500.

STK4 antibody detects STK4 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: PC-3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STK4 protein stained by STK4 antibody (OAGA02136) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human STK4. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: STK4
• Gene Full Name: serine/threonine kinase 4
• Alias Symbols: KRS2, MST1, YSK3
• NCBI Gene Id: 6789
• Description of Target: The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
• Uniprot ID: Q13043