STK4 Antibody (OAGA00756)

Catalog No: OAGA00756

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
STK4 antibody
OAGA00756 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00756 diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MST1(OAGA00756) antibody at 1:500 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(OAGA00756) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

STK4 antibody immunoprecipitates STK4 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 50 ug HepG2 whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG C.
Immunoprecipitation of STK4 protein by 4 ug STK4 antibody (OAGA00756) 7.5 % SDS-PAGE
The immunoprecipitated STK4 protein was detected by STK4 antibody (OAGA00756) diluted at 1:500.
Anti-rabbit IgG was used as a secondary reagent

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. Supplied in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB, IP
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain
  Cellular Localization: Cytoplasm, Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STK4. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Rat (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows:
  WB: 1:500-1:3000,
  IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)],
  ICC/IF: 1:100-1:1000.
  IP: 1:100-500.
  Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: STK4
• Gene Full Name: Serine/threonine-protein kinase 4
• Alias Symbols: KRS2, MST1, YSK3
• NCBI Gene Id: 6789
• Protein Name: Serine/threonine-protein kinase 4
• Description of Target: The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq]
• Uniprot ID: Q13043
• Protein Accession #: NP_006273.1
• Molecular Weight: 56 kDa