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Datasheets/Manuals | Printable datasheet for STK3 Antibody (OACD07546) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Range: Thr235 - Ala460 |
Purification | Affinity Purified; Protein A Purified |
Concentration | 0.5 mg/ml |
Preservative | 0.02% NaN3 |
Specificity | The antibody is a rabbit polyclonal antibody raised against STK3. It has been selected for its ability to recognize STK3 in immunohistochemical staining and western blotting. |
Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
Gene Symbol | Stk3 |
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Gene Full Name | serine/threonine kinase 3 |
Alias Symbols | 0610042I06Rik;mammalian STE20-like protein kinase 2;mes;mess1;MS;MST;Mst2;MST-2;Mst3;protein kinase homolog;serine/threonine-protein kinase 3;Ste2;Ste20;STE20-like kinase MST2. |
NCBI Gene Id | 56274 |
Protein Name | Serine/threonine-protein kinase 3 |
Description of Target | Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (By similarity). |
Uniprot ID | Q9JI10 |
Protein Accession # | NP_062609.2 |
Nucleotide Accession # | NM_019635 |
Molecular Weight | 49 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "STK3 Antibody (OACD07546)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "STK3 Antibody (OACD07546)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "STK3 Antibody (OACD07546)" provided in?
This item is provided in "Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "STK3 Antibody (OACD07546)"?
This target may also be called "0610042I06Rik;mammalian STE20-like protein kinase 2;mes;mess1;MS;MST;Mst2;MST-2;Mst3;protein kinase homolog;serine/threonine-protein kinase 3;Ste2;Ste20;STE20-like kinase MST2." in publications.
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What is the shipping cost for "STK3 Antibody (OACD07546)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "STK3 Antibody (OACD07546)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "STK3 Antibody (OACD07546)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "49 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "STK3 Antibody (OACD07546)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Stk3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Stk3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Stk3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Stk3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Stk3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Stk3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.