STK29 Antibody - C-terminal region (OAAB16966)

Catalog No: OAAB16966

Size:400ul

Western blot analysis of anti-STK29 Pab(Cat. # OAAB16966) in HL60 cell line lysate (35ug/lane). STK29(arrow) was detected using the purified Pab (1:60 dilution).

BRSK2 is strongly supported by BioGPS gene expression data to be expressed in HL60

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: STK29 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-260 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmatic reticulum (ER) stress
  Cellular Location: Cytoplasm, cytoskeleton, centrosome. Cytoplasm, perinuclear region. Endoplasmic reticulum Note=Detected at centrosomes during mitosis. Localizes to the endoplasmic reticulum in response to stress caused by tunicamycin
  Tissue Location: Detected in pancreas islets (at protein level)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This STK29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-736 amino acids from the C-terminal region of human STK29.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  BRSK2 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: BRSK2
• Alias Symbols: SAD1, SADA, STK29, PEN11B, C11orf7
• NCBI Gene Id: 9024
• Protein Name: Serine/threonine-protein kinase BRSK2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q8IWQ3
• Protein Accession #: NP_001243556.1
• Molecular Weight: 81633 Da