STK24 Antibody (OAGA01006)

Catalog No: OAGA01006

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
OAGA01006 diluted at 1:1000

STK24 antibody [N2C1], Internal detects STK24 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
STK24 antibody [N2C1], Internal (OAGA01006) dilution: 1:250.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STK24(OAGA01006) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC, WB
• Additional Information: Background: The yeast 'Sterile 20' gene (STE20) functions upstream of the mitogen-activated protein kinase (MAPK) cascade. In mammals, protein kinases related to STE20 can be divided into 2 subfamilies based on their structure and regulation. Members of the PAK subfamily (see PAK3; MIM 300142) contain a C-terminal catalytic domain and an N-terminal regulatory domain that has a CDC42 (MIM 116952)-binding domain. In contrast, members of the GCK subfamily (see MAP4K2; MIM 603166), also called the Sps1 subfamily, have an N-terminal catalytic domain and a C-terminal regulatory domain without a CDC42-binding domain. STK24 belongs to the GCK subfamily of STE20-like kinases (Zhou et al., 2000 [PubMed 10644707]).[supplied by OMIM]
  Positive Controls: 293T, A431, H1299, HeLa, Raji, rat brain
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 137 and 231 of MST3 (Uniprot ID#Q9Y6E0)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.94 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: STK24
• Gene Full Name: Serine/threonine-protein kinase 24
• Alias Symbols: MST3, STK3, MST3B, STE20, HEL-S-95
• NCBI Gene Id: 8428
• Protein Name: Serine/threonine-protein kinase 24
• Uniprot ID: Q9Y6E0
• Protein Accession #: NP_001027467.2
• Molecular Weight: 48 kDa