STK16 Antibody - C-terminal region (OAAB17023)

Catalog No: OAAB17023

Size:400ul

Western blot analysis of anti-STK16 Pab (Cat. # OAAB17023) in mouse brain tissue lysate. STK16 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of STK16 (arrow) using rabbit polyclonal STK16 Antibody (M272) (Cat. # OAAB17023). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STK16 gene.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: STK16 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins
  Cellular Location: Cytoplasm, perinuclear region. Membrane; Lipid-anchor (By similarity). Note=Associates with Golgi and Golgi-derived vesicles (By similarity)
  Tissue Location: Ubiquitously expressed at very low levels.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This STK16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human STK16.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: STK16
• Alias Symbols: PSK, KRCT, MPSK, TSF1, hPSK, PKL12
• NCBI Gene Id: 8576
• Protein Name: Serine/threonine-protein kinase 16
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O75716
• Protein Accession #: NP_001008910.1
• Molecular Weight: 34656 Da