Stk11 ELISA Kit (Rat) : 96 Wells (OKEH02287)

Catalog No: OKEH02287

Size:96T

Rat Stk11 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Stk11 ELISA Kit (Rat) (OKEH02287) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Stk11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Stk11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Stk11 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 10.7% (n = 20)
• ELISA Kit Component:
  • Stk11 Microplate: 96 Wells (12 x 8 Well strips)
  • Stk11 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated TTPA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Rat Serine/threonine-protein kinase STK11
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Stk11
• Gene Full Name: serine/threonine kinase 11
• Alias Symbols: liver kinase B1 homolog;Lkb1;serine/threonine-protein kinase 11;serine/threonine-protein kinase LKB1;serine/threonine-protein kinase STK11.
• NCBI Gene Id: 314621
• Protein Name: D4AE59
• Description of Target: Serine/threonine-protein kinase STK11
• Uniprot ID: https://www.uniprot.org/uniprot/D4AE59
• Protein Accession #: NP_001101539.1
• Nucleotide Accession #: NM_001108069.1
• Protein Size (# AA): 436
• Molecular Weight: 49 kDa