STC1 ELISA Kit (Human) (OKCD00763)

Catalog No: OKCD00763 (Formerly GWB-KBBZO9)

Size:96WELLS

Human STC1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	96-105%	82-89%	82-96%	88-98%
  EDTA plasma(n=5)	90-105%	90-97%	87-101%	84-91%
  heparin plasma(n=5)	89-101%	91-105%	80-93%	87-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stanniocalcin 1 (STC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stanniocalcin 1 (STC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stanniocalcin 1 (STC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Stanniocalcin 1 (STC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	97-105	101
  EDTA plasma(n=5)	86-96	93
  heparin plasma(n=5)	87-95	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stanniocalcin 1 (STC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stanniocalcin 1 (STC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-STC1 Microplate: 96 Wells (12 x 8 Well Strips)
  • STC1 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated STC1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 11.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidylglycine Alpha Amidating Monooxygenase (PAM). No significant cross-reactivity or interference between Peptidylglycine Alpha Amidating Monooxygenase (PAM) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: STC1
• Gene Full Name: stanniocalcin 1
• Alias Symbols: stanniocalcin-1;STC.
• NCBI Gene Id: 6781
• Protein Name: Stanniocalcin-1
• Description of Target: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.
• Uniprot ID: P52823
• Protein Accession #: NP_003146
• Nucleotide Accession #: NM_003155.2
• Protein Size (# AA): 247