Human STAT4 Antibody (Phospho-Tyr693) (OAAQ00167)

Catalog No: OAAQ00167

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.01 ug/mL of Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 Cat. #OAAQ00167 or Company C at 0.1 ug/mL (manufacturer's recommended concentration).

Flow cytometric analysis of K562 cells unstained and treated with imatinib as negative control (blue) or treated with imatinib and stained (red) or treated with IFNα + IL-4 + pervanadate and stained (green) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.01 ug/mL. Cat #OAAQ00167.

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or untreated (grey) or IFNα + IL-4 + pervanadate-treated (orange) using 0.1 ug/mL isotype control Cat. #OAAQ00101 or untreated (red) or treated (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.1 ug/mL. Cat. #OAAQ00167.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.1 ug/mL. Cat #OAAQ00167.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.01 ug/mL of Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 Cat. #OAAQ00167 or Company C at 0.1 ug/mL (manufacturer's recommended concentration).

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or untreated (grey) or IFNα + IL-4 + pervanadate-treated (orange) using 0.1 ug/mL isotype control Cat. #OAAQ00101 or untreated (red) or treated (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.1 ug/mL. Cat. #OAAQ00167.

Flow cytometric analysis of K562 cells unstained and treated with imatinib as negative control (blue) or treated with imatinib and stained (red) or treated with IFNα + IL-4 + pervanadate and stained (green) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.01 ug/mL. Cat #OAAQ00167.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat4 (Tyr693) antibody Stat4Y693-F6 at 0.1 ug/mL. Cat #OAAQ00167.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA.
• Clonality: Monoclonal
• Clone: Stat4Y693-F6
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: Unconjugated
• Application: FC
• Reconstitution and Storage: -20C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr693 of human phospho Stat4
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Tyr693
• Application Info: 1ug/mL - 0.001ug/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reference: Kaplan MH, Sun Y, Hoey T, and Grusby MJ. (1996) Nature. 382: 174-177.
  Chang H, Zhang S, Oldham I, Naeger L, Hoey T, and Kaplan MH. (2003) Journal of Biological Chemistry. 278: 32471-32477.

Target Info

• Gene Symbol: STAT4
• Gene Full Name: signal transducer and activator of transcription 4
• Alias Symbols: SLEB11
• NCBI Gene Id: 6775
• Protein Name: signal transducer and activator of transcription 4
• Description of Target: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.
• Uniprot ID: Q14765
• Protein Accession #: NP_001230764.1
• Nucleotide Accession #: NM_001243835.1