Stat3 ELISA Kit (Mouse) (OKBB01197)

Catalog No: OKBB01197

Size:96 wells

Stat3 ELISA Kit (Mouse) (OKBB01197) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse AAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AAT has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: E25-K413） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse AAT amount of sample captured in plate.
• ELISA Kit Range: 1.56 ng/ml - 100 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	2876	13987	37231	2911	14122	4269
  Standard deviation	158.18	713.3	1600.9	177.57	932.05	320.18
  CV(%)	5.5	5.1	4.3	6.1	6.6	7.5

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse Stat3 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse Stat3 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse Stat3 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	2876	2791	2438	2616	2680	168.37	6.2%
  Sample . 2	13987	13721	13461	14393	13890	344.60	2.4%%
  Sample 3	37231	37794	38247	41965	38809	1857.18	4.7%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (ng/ml)	0	1.56	3.13	6.25	12.5	25	50	100
  O.D.	0.042	0.207	0.368	0.659	1.054	1.369	1.645	2.069

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 15 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse AAT
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E25-K413
• Description: For quantitative detection of mouse AAT in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: STAT3
• Gene Full Name: signal transducer and activator of transcription 3
• Alias Symbols: Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; 17 kDa heparin-binding growth factor-binding protein; 17 kDa HBGF-binding protein; HBp17; FGFBP1; FGFBP; HBP17
• NCBI Gene Id: 20848
• Protein Name: Alpha-1-antitrypsin 1-2
• Description of Target: SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
• Uniprot ID: P22599