- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for STAT3 Antibody (Phospho-Tyr705) (OAAF00384) |
---|
Predicted Species Reactivity | Human|Mouse|Porcine|Rat |
---|---|
Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
Additional Information | Modification Sites: H: Y705, M: Y705, R: Y705 |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human STAT3 around the phosphorylation site of Tyr705. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho |
Specificity | STAT3 (Phospho-Tyr705) Antibody detects endogenous levels of STAT3 only when phosphorylated at Tyr705. |
Application Info | WB 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 Optimal dilutions should be determined by the end user. |
Gene Symbol | STAT3 |
---|---|
Gene Full Name | signal transducer and activator of transcription 3 |
Alias Symbols | acute-phase response factor;ADMIO;ADMIO1;APRF;DNA-binding protein APRF;HIES;signal transducer and activator of transcription 3. |
NCBI Gene Id | 6774 |
Protein Name | Signal transducer and activator of transcription 3 |
Description of Target | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). |
Uniprot ID | P40763 |
Protein Accession # | NP_003141.2 |
Molecular Weight | 88 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Porcine|Rat".
-
How long will it take to receive "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)"?
This target may also be called "acute-phase response factor;ADMIO;ADMIO1;APRF;DNA-binding protein APRF;HIES;signal transducer and activator of transcription 3." in publications.
-
What is the shipping cost for "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "88 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "STAT3 Antibody (Phospho-Tyr705) (OAAF00384)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "STAT3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "STAT3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "STAT3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "STAT3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "STAT3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "STAT3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.