STAT1 ELISA Kit (Human) (OKEH08419)

Catalog No: OKEH08419

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology STAT1 ELISA Kit (Human) (OKEH08419) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for STAT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for STAT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample STAT1 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.4% (n = 20)
  Mean Inter-assay CV%: <=11.3% (n = 20)
• ELISA Kit Component:
  • STAT1 Microplate: 96 Wells (12 x 8 Well strips)
  • STAT1 Lyophilized Standard: 2
  • 100X Biotinylated STAT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
  Subcellular Location: Cytoplasm Nucleus Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4.
  Unigene: Hs.642990
  SMR: P42224
  String: 9606.ENSP00000354394
  KEGG: hsa:6772
  MIM: 600555
  Pfam: PF00017
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 15.61pg/mL
• Specificity: Natural and recombinant Human Signal transducer and activator of transcription 1-alpha/beta
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: STAT1
• Gene Full Name: signal transducer and activator of transcription 1
• Alias Symbols: CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;signal transducer and activator of transcription 1, 91kD;signal transducer and activator of transcription 1, 91kDa;signal transducer and activator of transcription 1-alpha/beta;STAT91;transcription factor ISGF-3 components p91/p84.
• NCBI Gene Id: 6772
• Protein Name: P42224
• Description of Target: Signal transducer and activator of transcription 1-alpha/beta
• Uniprot ID: https://www.uniprot.org/uniprot/P42224
• Protein Accession #: NP_009330.1
• Nucleotide Accession #: NM_007315.3