STAT1 Antibody (OAEE00534)

Catalog No: OAEE00534

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: SM2
• Isotype: IgG1
• Host: Mouse
• Application: Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: STAT1 peptide sequence 8-23 (QLDSKFLEQVHQLYD) conjugated to KLH.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody SM2 recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Kovarik P, Stoiber D, Eyers PA, Menghini R, Neininger A, Gaestel M, Cohen P, Decker T.: Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13956-61.
  Frank D.A.: STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56.
  Bromberg J, Darnell J.E. Jr: The role of STATs in transcriptional control and their impact on cellularfunction. Oncogene. 2000 May 15;19(21):2468-73.
  Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A.: ERK and the E3 ubiquitin ligase beta TRCP targets STAT1 for degradation. J Biol Chem. 2008 Mar 31

Target Info

• Gene Symbol: STAT1
• Gene Full Name: signal transducer and activator of transcription 1
• Alias Symbols: CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;signal transducer and activator of transcription 1, 91kD;signal transducer and activator of transcription 1, 91kDa;signal transducer and activator of transcription 1-alpha/beta;STAT91;transcription factor ISGF-3 components p91/p84.
• NCBI Gene Id: 6772
• Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• Description of Target: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
• Uniprot ID: P42224
• Protein Accession #: NP_009330.1; NP_644671.1; XP_006712781.1
• Nucleotide Accession #: NM_007315.3; NM_139266.2; XM_006712718.1