STAM Antibody (OAGA00446)

Catalog No: OAGA00446

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
OAGA00446 diluted at 1:3000

Immunohistochemical analysis of paraffin-embedded AdCA xenograft, using STAM(OAGA00446) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: This gene was identified by the rapid tyrosine-phosphorylation of its product in response to cytokine stimulation. The encoded protein contains a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). This protein associates with JAK3 and JAK2 kinases via its ITAM region, and is phosphorylated by the JAK kinases upon cytokine stimulation, which suggests the function of this protein is as an adaptor molecule involved in the downstream signaling of cytokine receptors. HGS/HRS (hepatocyte grwoth factor-regulated tyrosine kinase substrate) has been found to bind and counteract the function of this protein. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
  Cellular Localization: Cytoplasm (Probable), Early endosome membrane; Peripheral membrane protein; Cytoplasmic side
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 16 and 326 of STAM (Uniprot ID#Q92783)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: STAM
• Gene Full Name: Signal transducing adapter molecule 1
• Alias Symbols: STAM1, STAM-1
• NCBI Gene Id: 8027
• Protein Name: Signal transducing adapter molecule 1
• Uniprot ID: Q92783
• Protein Accession #: NP_003464.1
• Molecular Weight: 59 kDa