Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH05820
Size:96T
Price: $525.00
SKU
OKEH05820
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Stab2 ELISA Kit (Mouse) (OKEH05820) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Stab2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Stab2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Stab2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 100.7%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <6.2%, n = 20
Mean Inter-Assay Precision - <8.6%, n = 20
ELISA Kit Component
ComponentAmount
STAB2 Microplate96 Wells (12 x 8 Well strips)
STAB2 Lyophilized Standard2
100X Biotinylated STAB2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.159 ng/mL
SpecificityNatural and recombinant Mouse Stabilin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolStab2
Gene Full Namestabilin 2
Alias Symbolsfasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavenger receptor 2;FEEL-;FEEL-2;FELL;MFEEL-2;scavenger receptor FEEL-2;STAB;STAB-2;stabilin-2.
NCBI Gene Id192188
Protein NameQ8R4U0
Description of TargetStabilin-2
Uniprot IDhttps://www.uniprot.org/uniprot/Q8R4U0
Protein Accession #NP_619614.1
Nucleotide Accession #NM_138673.2
Protein Size (# AA)2559
Write Your Own Review
You're reviewing:STAB2 ELISA Kit (Mouse) (OKEH05820)
Your Rating
We found other products you might like!