ST3GAL1 Antibody (OAGA00459)

Catalog No: OAGA00459

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:HeLa S3
12% SDS PAGE
OAGA00459 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded serous OVCA, using ST3GAL1(OAGA00459) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed Hep3B, using SIAT4A(OAGA00459) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3
  Cellular Localization: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein, Secreted
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 195 of SIAT4A (Uniprot ID#Q11201)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.98 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ST3GAL1
• Gene Full Name: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
• Alias Symbols: ST3O, SIAT4A, SIATFL, ST3GalA, ST3GalIA, Gal-NAc6S, ST3GalA.1, ST3GalIA,1
• NCBI Gene Id: 6482
• Protein Name: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
• Uniprot ID: Q11201
• Protein Accession #: NP_003024.1
• Molecular Weight: 39 kDa