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Catalog No: OCOA11008
Size:20UG
Price: $275.00
SKU
OCOA11008
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ST18 Protein Lysate (OCOA11008)

Datasheets/ManualsPrintable datasheet for ST18 Protein Lysate (OCOA11008)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionST18 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolST18
Gene Full NameST18 C2H2C-type zinc finger transcription factor
Alias Symbolsneural zinc finger transcription factor 3;NZF3;NZF-3;ST18, C2H2C-type zinc finger;suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein);suppression of tumorigenicity 18 protein;suppression of tumorigenicity 18, zinc finger;ZC2H2C3;ZC2HC10;zinc finger protein 387;ZNF387.
NCBI Gene Id9705
Protein NameNM_014682, NP_055497, O60284
Description of TargetHomo sapiens ST18, C2H2C-type zinc finger (ST18), transcript variant 1, mRNA, Suppression of tumorigenicity 18 protein, suppression of tumorigenicity 18 protein isoform a [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/O60284
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_055497
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_014682
Molecular Weight115 kDa
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